High-resolution structure of eukaryotic Fibrillarin interacting with Nop56 amino-terminal domain

Structural comparison of Sc Nop1^83–327 and Nop56^1–166 to their archaeal orthologs Fibrillarin and Nop5. (A, left) Structure of Nop1^83–327 with the small amino-terminal and central domains highlighted. The active site is indicated with red dashed lines. The structure is part of the Sc Nop56^1–166–Nop1^83–327 complex structure determined here (see Fig. 4). (Right) Superposition of the structure of Nop1^83–327 with those of archaeal orthologs. Sc Nop1^83–327 is in slate, Pf Fibrillarin (PDB-ID: 2NNW) in green, Ss Fibrillarin (PDB-ID: 3ID5) in forest, and Af Fibrillarin (PDB-ID: 1NT2) in smudge. (B, left) Structure of Nop56^1–166 with secondary structure elements indicated. The structure is part of the Sc Nop56^1–166–Nop1^83–327 complex structure determined here (see Fig. 4). (Right) Superposition of the structure of Sc Nop56^1–166 with those of Nop5 amino-terminal domains from archaea. Sc Nop56^1–166 is in green cyan, Pf Nop5^1–123 (PDB-ID: 2NNW) in hot pink, Ss Nop5^1–132 (PDB-ID: 3ID5) in magenta, and Af Nop5^1–71 (PDB-ID: 1NT2) in violet purple. RMSD values were calculated between all heavy atoms.