High-resolution structure of eukaryotic Fibrillarin interacting with Nop56 amino-terminal domain

Homology model of Sc Nop58 amino-terminal domain. (A) Homology model generated with MODELLER (Webb and Sali 2016) using the high-resolution structure of Nop56^1–166 in the Nop56^1–166–Nop1^83–327 as template. The secondary structure elements are indicated. (B) Surface electrostatics of the Nop1-binding interface of the Nop58^1–155 homology model (top) and the Nop56^1–166 structure (bottom). White, blue and red indicate neutral, positive, or negative surface charges. (C) Residues of the Nop1-binding interface conserved in Nop56 and Nop58 mapped on the Nop58^1–155 homology model. The eukaryotic-specific insertion between β2 and β3 of the Nop58 amino-terminal domain is in beige.