FIGURE 1.
tRNA annotations in planaria. (A) Bioinformatic pipeline used to annotate planarian tRNAs. Two different prediction algorithms were used to identify a confident set of 457 tRNAs in S.mediterranea. (B) Median lengths of tRNA identified in S. mediterranea. (C) Bar graph depicting the position of anticodons across planarian tRNAs. (D) The gene copy numbers of all the identified planarian tRNAs across S. mediterranea genome. (E) Conserved features of i-Met tRNAs identified in planaria as compared to the S. cerevisae iMet tRNA. The colors indicate the conserved sequence signatures. (F) Northern hybridization blot validation of predicted tRNAs (shown in duplicates). The arrow points to the tRNA band.
