Alternative RNA degradation pathways by the exonuclease Pop2p from Saccharomyces cerevisiae

Processive RNA degradation by Pop2p. (A) Reaction scheme for pulse-chase experiments. (B) Representative PAGE for a pulse-chase reaction of Pop2p (1200 nM) with A₃₆ (1 nM) and scavenger RNA (5 µM). (t₁: reaction time after addition of labeled substrate. t₂: incubation time after addition of scavenger RNA). (C) Processivity values of Pop2p for A₃₆ and A₂₄ RNA substrates. Data points represent the average of three independent experiments, error bars show one standard deviation. The average number of steps (right axis) was calculated according to P = (N − 1)N⁻¹ (P: processivity value, N: average number of steps) (Axhemi et al. 2020).