Ribosome bound Ebp1 can undergo a rotation around the insert domain. (A) Overview of Ebp1 binding site on human ribosome obtained in this study is shown in two orientations. Ebp1 (green), eL19 (yellow), uL23 (magenta), uL24 (orange), uL29 (wheat) proteins, fragment of 5.8S rRNA (purple), and different regions (H53, H59, and ES27L) of the 28S rRNA (light blue) are shown as cartoon. (B) Overview of Ebp1 binding site on human ribosome from a previous study is shown in two orientations (PDB: 6SXO [Wild et al. 2020]). Ebp1 (light pink), eL19 (yellow), uL23 (magenta), uL24 (orange), uL29 (wheat) proteins, fragment of 5.8S rRNA (purple), and different regions (H53, H59, and ES27L) of the 28S rRNA (light blue) are shown as cartoon (as in A). (C) Relative orientation of Ebp1 (green and light pink) with respect to 60S subunit is shown based on superposition of ribosomal proteins in two orientations. The axis and degree of rotation of Ebp1 are indicated. Arrows (gray) indicate a few regions of Ebp1 that undergo conformational change.
