Comparison of the human Ebp1-80S and yeast Arx1-60S complexes (Greber et al. 2016). (A) Superposition of yeast Arx1-ribosome complex (PDB: 5APN [Greber et al. 2016]) on Ebp1-ribosome complex. Ebp1 (green), Arx1 (dark beige), eL19 (yellow), uL23 (magenta), uL24 (orange), uL29 (wheat) proteins, fragment of 5.8S rRNA (purple), and different regions (H53, H59, and ES27L) of the 28S/25S rRNA (light blue and blue) are shown as cartoon. Insert domains of Ebp1 and Arx1 are highlighted with a box. (B) Rotation of ES27L in human Ebp1 (green)-ribosome complex in comparison with yeast Arx1 (dark beige)-60S complex. Only 28S/25S rRNA of human and yeast ribosome are displayed (light blue and blue) for clarity. (C) Additional insertion regions of yeast Arx1 (dark beige) that mediate interaction with uL24 (orange), uL29 (wheat), 5.8S rRNA (purple), and H24 of 28S/25S rRNA are indicated with boxes. Other proteins and rRNA fragments are colored as in A.
