The effect of SHP2 deletion on growth plate development. (A) Colocalization of the lineage tracing reporter signal and the RNAscope signal identified the Ihh (red) mRNA expressed in the prehypertrophic zone of growth plates of tibias from 10-wk-old SHP2CTRAgc1/ER and SHP2KOAgc1/ER mice. (B) Measurement of the thickness of Ihh-expressing cell layer (indicated by the double-headed arrow) in the middle region of the growth plates demonstrated significant increase in Ihh mRNA expression in the growth plates of mutant mice compared to those of the controls (n = 5) (***) P < 0.001). The borders of the RNAscope signal-positive region were outlined with dashed lines. The length of the scale bar represents 50 µm.
