Detection of knockout efficiency of the target gene at the mRNA level in reporter-positive cells in vivo. Tibial bones from 10-wk-old mice were used. (A) DAPI, ZsG, and Shp2 mRNAs are indicated by the blue, green, and red dots, respectively. A single red dot represents at least one Shp2 mRNA. (B) There was a significant reduction (−77%) in the number of the red dots in 100 R26ZsG+ chondrocytes from the middle region of growth plates in SHP2KOAgc1/ER mice compared to the controls (n = 5, [***] P < 0.0001). Enlarged views of the boxed areas were shown at the bottom left corner of each image. Shp2 mRNAs are represented as small red dots marked by white arrows in the enlarged views. The length of the scale bar represents 50 µm.
