Colocalization of lineage tracing reporter signal and RNAscope signal on the same skeletal section. Tibial bones of 6-wk-old SHP2CTRAgc1/ER mice were used. (A) DAP1 (blue) and ZsG (green) reporter images of growth plate chondrocytes exhibited strong ZsG reporter signals due to Agc1-Cre. (B) Sox9, Ihh, and Col10a1 mRNA (red) were respectively expressed in the proliferating, prehypertrophic, and hypertrophic chondrocytes of growth plates. (C) Each merged figure displays the colocalization of the ZsG reporter signal and the Sox9, Ihh, or Col10a1 mRNA signal. The borders of the RNAscope signal-positive regions are outlined with dashed lines. The length of the scale bar represents 50 µm.
