CtIP depletion affects gene expression and splicing of many genes. (A) Distribution of the genes up-regulated upon CtIP depletion regarding the exposure or not to DNA damage. Fold change (FC) > 2, P-value < 0.05. Gene expression was measured using the GeneChip HTA Array as described in the Materials and Methods section. The number of up-regulated genes in undamaged cells (green), 6 h after exposure to irradiation (10 Gy; pink) or both (yellow) is shown in a Venn diagram. The actual list of genes can be found in Table 4. (B) Same as A but for down-regulated genes. The actual list of genes can be found in Table 5. (C) The GeneChip HTA Array was used to look for genes that changed their splicing upon SF3B2 and/or CtIP depletion, as mentioned in the Materials and Methods section. The Venn diagram represents the one that changes when CtIP (pink), SF3B2 (green), or both are down-regulated. Other details as in A. (D) Differential splicing events bteween different conditions: undamaged cells depleted for CtIP (siCtIP) or transfected with a nontarget siRNA (siNT); or RNA collected 6 h after irradiation in cells depleted for CtIP (siCtIP_6h) or control cells (siNT_6h).
