Inducible nuclear import by TetR aptamer-controlled 3′ splice site selection

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FIGURE 3.
FIGURE 3.

Microscopic visualization of inducible control of nuclear import with a TetR aptamer. (A) A3′SS-TN2-CP stably integrated into an HF1-3 cell line using the Flp-In system. (B,C) The generated cell line expressing the A3′SS-TN2-CP construct was transiently transfected with mCherry-tagged TetR (+) and treated with (+) or without (−) 50 µM doxycycline (dox) for 24 h. Cells were fixed and stained with DAPI. Scale bar, 10 µm.

This Article

  1. Published in Advance November 4, 2020, doi: 10.1261/rna.077453.120 RNA 27: 234-241

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