Posttranscriptional modifications at the 37th position in the anticodon stem–loop of tRNA: structural insights from MD simulations

(A) Probability distribution in the location of the side chain in the i⁶A hypermodified nucleobase at the 37th position in E. coli tRNA. (B) Overlays of i⁶A, io⁶A, or ms²i⁶A modified nucleobases in tRNA with respect to the nucleobase ring for structures taken at 5 ns intervals over the MD simulations. (C) Average structural deviation of anticodon nucleotides (RMSF, Å) over the MD simulations with respect to the initial structure for each i⁶A modified tRNA compared to unmodified A-tRNA.