Identification of CIRBP targets. (A) Schematic representation of our RIP-seq approach to identify CIRBP targets. (B) Efficiency of immunoprecipitation in duplicate experiments. Immunoprecipitations with nonspecific IgG were carried in parallel as control. (i) input, (P) pellet, (S) supernatant. (C) A target was considered positive when there was significant enrichment (L2FC > 1, Padj < 0.01) in the CIRBP IP versus the input, or in the CIRBP IP versus the IgG control. Targets positive in both analysis (204) were considered high-confident targets. Most of these targets are protein-coding genes (right). (D) Gene Ontology (GO) analysis of the high confidence targets. The top 20 biological processes were selected for representation. (E) Comparison between CIRBP high-confidence targets identified in this study and previous transcriptome-wide analysis of CIRBP targets.
