CIRBP promotes tumorigenic traits. (A) MCF-10A cells were stably transduced with a retroviral vector expressing CIRBP (+), or with the empty vector (−) as control. Western blots with anti-Flag (left) or anti-CIRBP (right) antibodies are shown. These cells were tested for proliferation (B) and clonogenicity (C). (D) MCF-7 cells were transiently transfected with siRNA pools against CIRBP or with a control siRNA pool. The efficiency of depletion was assessed by western blot at the beginning of the experiment (day 0). These cells were tested for proliferation (E) and clonogenicity (F). Error bars represent the standard deviation of triplicate experiments. Statistical significance was assessed by Student's t-test (*) P < 0.05, (**) P < 0.01.
