Synthesis of modified nucleotide polymers by the poly(U) polymerase Cid1: application to direct RNA sequencing on nanopores

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FIGURE 3.
FIGURE 3.

Ability of Cid1 to use modified UTP analogs for tailing. Cid1 tailing reactions were set up with the indicated analog either without (−, 1mM) or with (+, 0.5 mM each) competing unmodified UTP, using the MYL6(A+) model mRNA as a substrate. The 6% denaturing polyacrylamide gel was stained with SYBR Gold and imaged on a Typhoon Imager.

This Article

  1. Published in Advance August 26, 2021, doi: 10.1261/rna.078898.121 RNA 27: 1497-1511

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