Editing of miR-379 in ADAR-overexpressing PC3 cells. (A) ADAR and ADARB1 mRNA expression levels in PC3 cells transduced with ADAR overexpression or control vectors. Relative expression values were calculated using the geometric mean of GUSB and PGK1 mRNAs for normalization. (B) Western blot showing ADAR1 and ADAR2 protein expression in PC3 cells transduced with ADAR overexpression or control vectors. GAPDH is the loading control. (C) miR-379 editing frequency in PC3 cells transduced with ADAR overexpression or control vectors. Editing frequencies were calculated based on the absolute number of edited miR-379 molecules divided by the sum of unedited and edited miR-379 molecules. Absolute numbers were derived from standard curves of serially diluted RNA oligonucleotides. (D) Editing frequencies of pri-miR-379 in PC3 cells transduced with ADAR overexpression or control vectors, as determined by RT-PCR and Sanger sequencing.
