Quantification of microRNA editing using two-tailed RT-qPCR for improved biomarker discovery

Detection of miR-379 editing isoforms with commercial TaqMan assays. (A) C_t values for different dilutions of unedited (red circles) and edited (blue squares) miR-379 RNA oligonucleotides quantified with TaqMan Advanced microRNA assays. PCR efficiencies were calculated based on the slope. Standard deviations were too small to be plotted as error bars. (B) Relative detection of edited miR-379 compared to an equal number of unedited miR-379 molecules as calculated from the difference in C_t cycles in A.