Quantification of in vitro ESKAPE trans-translation and translation assays after CT1-83 and KKL-35 treatment. Experiments were conducted in microplates, using ESKAPE homologous systems and increasing concentrations (50, 100, and 200 µM) of CT1-83 and KKL-35 oxadiazole compounds. Normalized fluorescence intensities obtained are presented after 310 min of incubation and sorted by species. The results were normalized to the neutral control conditions and are shown as means ± standard deviations. (A) Trans-translation assay with the oxadiazole compounds CT1-83 and KKL-35. The positive control was obtained by using 10 µM Antisense B in 10% DMSO. (B) Translation assay with the oxadiazole compounds CT1-83 and KKL-35. The positive control was obtained by using Chloramphenicol at 100 µM in 10% DMSO. Using the Anova test, results were considered statistically significant when P ≤ 0.01. Only positive controls presented a difference of P ≤ 0.0001 for translation and trans-translation (stars were not represented to facilitate graph reading).
