Safe and easy in vitro evaluation of tmRNA-SmpB-mediated trans-translation from ESKAPE pathogenic bacteria

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FIGURE 3.
FIGURE 3.

Translation and trans-translation kinetics over time. (A) Translation kinetics over time: the increase in fluorescence of full-length GFP (i.e., encompassing the 12 beta-strands) is directly linked to translation. (B) Trans-translation kinetics over time using ESKAPE ribosomes. All results are shown as means ± standard deviation and normalized to E. coli.

This Article

  1. Published in Advance August 5, 2021, doi: 10.1261/rna.078773.121 RNA 27: 1390-1399

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