Safe and easy in vitro evaluation of tmRNA-SmpB-mediated trans-translation from ESKAPE pathogenic bacteria

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FIGURE 2.
FIGURE 2.

Trans-translation kinetics over time using Escherichia coli ribosomes. Fluorescence increases are directly linked to trans-translation activity. (A) Trans-translation assays were done on E. coli tmRNAGFP11 using the SmpBs from each ESKAPE pathogen, with the E. coli SmpB as a control. (B) Trans-translation assays keeping the E. coli SmpB but using the tmRNAGFP11 variants of each ESKAPE pathogen, with an E. coli tmRNAGFP11 as the control. (C) Both SmpB and tmRNAGFP11 are from each ESKAPE pathogen, with the E. coli SmpB-tmRNAGFP11 as a control. The results are shown as means ± standard deviation and normalized to the E. coli conditions.

This Article

  1. Published in Advance August 5, 2021, doi: 10.1261/rna.078773.121 RNA 27: 1390-1399

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