Safe and easy in vitro evaluation of tmRNA-SmpB-mediated trans-translation from ESKAPE pathogenic bacteria

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FIGURE 1.
FIGURE 1.

(A) Trans-translation of sfGFP1-10 mRNA lacking a stop codon. The tmRNAGFP11-SmpB complex binds to the stalled ribosome, and translation resumes thanks to the tmRNAGFP11 mRNA-like domain (MLD). The MLD encodes the missing eleventh beta-strand of the sfGFP, and the complete sfGFP is released and becomes fluorescent. (B) Impairment of the process in the presence of trans-translation inhibitors. The ribosomes stay stalled on the problematic mRNA and fluorescence is impaired.

This Article

  1. Published in Advance August 5, 2021, doi: 10.1261/rna.078773.121 RNA 27: 1390-1399

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