Identification and characterization of VapBC toxin–antitoxin system in Bosea sp. PAMC 26642 isolated from Arctic lichens

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FIGURE 4.
FIGURE 4.

Growth inhibition by BoVapC1 and its recovery by BoVapB1. (A) Neutralization of BoVapC1-mediated growth inhibition by BoVapB1. BL21(DE3) cells harboring pET21c-BoVapB1 and pBAD33-BoVapC1 were cultivated as described in Materials and Methods, and OD600 values were measured. Red arrow indicates induction starting point. (B) Neutralization of BoVapC1 by BoVapB1 mutants. The BL21(DE3) cells pretransformed with pET21c, pET21c-BoVapB1, or pET21c-BoVapB1 mutants were double transformed with pBAD33 or pBAD33-BoVapC1. The neutralization assay was conducted as described in Figure 2. A; cells with pBAD33/pET21c, B; pBAD33-BoVapC1/pET21c, C; pBAD33/pET21c-BoVapB1, and D; pBAD33-BoVapC1/pET21c-BoVapB1.

This Article

  1. Published in Advance August 24, 2021, doi: 10.1261/rna.078786.121 RNA 27: 1374-1389

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