FIGURE 4.
KDM3A allows for SRSF3 binding to SAT1 pre-mRNA. (A) MCF7 cells were transfected with nontargeting siRNA (siNT) or siSF3B1, siSRSF1/3/6 and sihnRNPF for 72 h. Total RNA was extracted and analyzed by real-time PCR for SAT1 exon X inclusion. PSI was calculated by SAT1 exon X relative to SAT1 total mRNA amount. (B) RNA-IP of SRSF3 in MCF7 cells that were transfected with nontargeting siRNA (siNT) or siKDM3A for 72 h. Real-time PCR analysis of SAT1 intron 3 (near exon X) relative to input. Values represent averages of three independent experiments ± SD. (*) P < 0.05; (**) P < 0.01; (***) P < 0.001; (****) P < 0.0001.
