Impact of pseudouridylation, substrate fold, and degradosome organization on the endonuclease activity of RNase E

Proposed model for substrate recognition and processing by RNase E. RNase E mediated processing of RNAs within the degradosome assembly is sensitive to substrate entry and product exit where other degradosome proteins RhlB, Enolase, and PNPase play an important role. The endonuclease activity of RNase E is guided by side chain interaction with substrate and geometrical details including a hydration pattern that can be influenced by pseudouridine substitution.