Impact of pseudouridylation, substrate fold, and degradosome organization on the endonuclease activity of RNase E

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FIGURE 3.
FIGURE 3.

Metal interactions in the active site of RNase E. (A) Schematic of RNase E NTD showing mutant D346C in the active site. The mutant is catalytically active in the presence of Mn++ but not any other metal as seen for processing of 9S and sRNA RprA (B,C, respectively). (D) An isothermal calorimetry titration curve for NTD.D346C interactions with Mn++. The KD is 17 µM for Mn++. The titration curve is representative of three independent experiments.

This Article

  1. Published in Advance August 2, 2021, doi: 10.1261/rna.078840.121 RNA 27: 1339-1352

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