Unique anticodon loop conformation with the flipped-out wobble nucleotide in the crystal structure of unbound tRNA^Val

Structural comparison of modified and unmodified ASL^Val1. Anticodon loops of protomer B (salmon) and modified ASL^Val (teal, PDB code: 2UUA) are superposed. (A) Comparison of overall tertiary structures, where the phosphate backbone is shown as a tube. The distance between the 2′-OH of wobble uridines is labeled. (B) A close-up view of atomic interactions between U34 and A35, where an additional hydrogen bond is made between the N3 of U34 and the N7 of A35 in the modified ASL. Averaged distances of hydrogen bonds are labeled. (C) A close-up of A37/m⁶A37 and neighboring residues.