Unique anticodon loop conformation with the flipped-out wobble nucleotide in the crystal structure of unbound tRNA^Val

The overall structure of E. coli tRNA^Val1 (A) Cloverleaf secondary structure of E. coli tRNA^Val1, which was utilized in the present X-ray crystallographic study. (B) A ribbon representation of two NCS-related tRNA^Val1 molecules in the asymmetric unit cell, where protomer A is shown in blue and protomer B is in salmon. Mg²⁺ and Na⁺ are displayed in green and purple spheres, respectively. (C) Superposition of protomers A and B and identical color scheme is used as in B. (D) Close-up view of ASL domains from overlaid structures in C.