Genome-wide analyses of XRN1-sensitive targets in osteosarcoma cells identify disease-relevant transcripts containing G-rich motifs

XRN1 also regulates ncRNAs in SAOS-2 cells. (A) Assessment of transcript proportions affected by XRN1 depletion relative to the genome-wide proportions detected in our sequencing data. (B) Scatter plot of changes in expression of all ncRNAs detected in our sequencing data. HOTAIR highlighted in black, (gray) no change, (red) post-transcriptionally up-regulated, (orange) transcriptionally up-regulated, (purple) post-transcriptionally down-regulated, (blue) transcriptionally down-regulated, (green) no intron data. (C–E) Boxplots of (C) expression, (D) GC content, and (E) length (bp) of ncRNAs in our data. Grouped by their nature of change in expression and compared to the genome average as detected in our data set.