Mammalian conservation of the mGlu4 duplex. (A) Alignment of rat mGlu6, mGlu7, and mGlu8 mRNA sequences to the mGlu4 duplex. (B) Sanger sequencing data demonstrating a dual adenosine and guanosine chromatogram peak characteristic of RNA edited transcripts in mGlu4 samples amplified from rat cerebellum but not mGlu7 or mGlu8 transcripts amplified from rat hippocampus. (C) Alignment of the mGlu4 duplex across multiple species. Q124R and K129R codons altered by editing are outlined in red boxes. Nucleotides are colored by percent conservation. Darker blue represents higher % conservation of the highlighted nucleotide. Conservation demonstrated in four groups from darkest blue to white (>80%, >60%, >40, <40%). (D) Cladogram of mGlu4 duplex sequence generated using DNAstar software (DNASTAR Inc.). Percent identity is shown in comparison to the human sequence. Sequences producing a similar extended RNA duplex to human are noted by a check mark.
