EB1 binds RNA opportunistically in vivo. (A) RNA immunoprecipitation-seq experiment for EB1-GFP led to the enrichment of 1017 genes with P-value <0.01: DESeq2 analysis of mRNAs pulled by EB1-GFP and GFP as a control. In red are the genes that cross the threshold of P-value <0.01 and fold change >4. (B) Frequency of colocalization of 12 of the candidate RNAs with EB1-GFP in Drosophila oocytes. osk mRNA is used as a negative control; (C) Four of the candidate genes (chc, asp, msps, dhc64c) exhibit a particular pattern of localization in the oocyte. Germline specific EB1 knockdown (right panels) using RNAi revealed no change in the localization pattern of these genes as compared to the wild-type (left panels). A schematic of the Drosophila egg chamber (bottom).
