propiR1 regulates lnc027353 expression during early development. (A) Northern blot analysis of propiR1 in early embryonic development, with two different exposure times. Time indicates the age of the embryo in hours after a 30-min egg laying period (oviposition). For each time point, RNA from 200–400 eggs was pooled. The northern blot, previously used in Halbach et al. (2020), was reprobed with a propiR1 probe. The EtBr stained rRNA panel is shown here again as loading control, reproduced from Figure 3A in Halbach et al. (2020) (reproduced with permission from Halbach et al. 2020, © Springer Nature). (B) lnc027353 expression during early embryonic development, as measured by RT-qPCR. Line and dots represent mean and individual measurements of two technical replicates, respectively. Expression was normalized to the 0 h time point. The same RNA was used in A and B. (C) lnc027353 expression in embryos injected with propiR1 or control antisense oligonucleotides (AO), as measured by RT-qPCR. Per condition, ∼50 embryos were pooled. A representative of three independent experiments is shown. Bars and dots indicate the mean and individual measurement of technical triplicates, respectively.
