A real-time fluorescence assay for CPSF73, the nuclease for pre-mRNA 3′-end processing

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FIGURE 4.
FIGURE 4.

Characterization of human CPSF73 inhibition by JTE-607 and AN3661 by the real-time fluorescence assay. (A) Dose-response curve of the reaction velocity as a function of JTE-607 concentration. The curve shows the fitting to the data to determine IC50 value. (B) Dose-response curve of the reaction velocity as a function of AN3661 concentration. The point at 10 µM AN3661 was not included in the fitting. The error bars represent ±1 standard deviation from triplicate measurements.

This Article

  1. Published in Advance July 6, 2021, doi: 10.1261/rna.078764.121 RNA 27: 1148-1154

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