A real-time fluorescence assay for CPSF73, the nuclease for pre-mRNA 3′-end processing

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FIGURE 3.
FIGURE 3.

Characterization of the real-time fluorescence assay for CPSF73 activity. (A) Reaction velocities under various conditions. (All) A reaction containing all the components. (B) Plot of the reaction velocity as a function of H2a* concentration. The error bars represent ±1 standard deviation from triplicate measurements.

This Article

  1. RNA 27: 1148-1154