Functional significance of the predicted base-pairing interaction between the A-rich tract and the mascRNA 3′ trailer. Schematic diagrams show the 3′-terminal triple helix structure of mature MALAT1 (A) and the mascRNA tRNA-like structure with its predicted extended acceptor stem in the MALAT1 precursor (B). Colored boxes outline the nucleotides mutated in this study. (C) Northern blot analysis of β-globin and NeoR transcripts (top) and quantitation (bottom) were carried out as in Figure 1E. Wild-type (black) and mutated (red) nucleotides are shown in colored boxes as in A and B. The Δ symbol represents a nucleotide deletion. Relative accumulations, which are normalized relative to the wild-type construct, are the average of at least three independent experiments ± SD.
