Antisense inhibition of SNRNP70 IR and SPTA1 IR. (A) SNRNP70 gene structure in the IR region showing retained intron 7 (thick gray line) with its major decoy exon, together with adjacent introns and exons. Position of antisense MO designed to block the 5′ splice site is shown, along with primer pairs used for RT-PCR. (B) SPTA1 gene structure in the IR region showing retained intron 20 (thick gray line) with its major decoy exon, together with adjacent introns and exons. Position of antisense MO designed to block the 5′ splice site is shown, along with primer pairs used for RT-PCR. (C,D) IR in cells treated with SNRNP70-specific (C) or SPTA1-specific (D) MO, in parallel with cells subjected to control MO treatment. IR was assessed using RT-qPCR to compare the relative amounts of IR and spliced products.
