FIGURE 4.
Western blot analysis of proteins bound to mutant pre-18S RNAs. Wild-type (WT) or mutant pre-18S RNAs were expressed in Utp9-TAP strain. The RNPs assembled on plasmid-derived pre-18S RNAs were purified via the MS2-tag on RNA and the TAP-tag in Utp9. The input (IN) and immunoprecipitated (IP) proteins were resolved in SDS-PAGE and immunoblotted with antibodies against TAP-tag, Krr1, and Enp1. The 18S processing phenotype and the mutated region are indicated for each mutant.
