Role of the central junction in folding topology of the protein-free human U2–U6 snRNA complex

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FIGURE 5.
FIGURE 5.

Analysis of binding affinity of contruct WTΔL (A) and contruct 4HJΔL1 (B) to RBM22. Concentration of RNA was constant at 15 µM and RBM22 concentration varied from 6 µM to 22.5 µM, corresponding to the RNA:RBM22 ratio from 1:0.4 to 1:1.5. Binding curves were fitted by a one-site binding (hyperbola) model using Prism. Goodness of fit was evaluated by least squared fit R2 with 95% confidence intervals.

This Article

  1. Published in Advance March 27, 2020, doi: 10.1261/rna.073379.119 RNA 26: 836-850

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