Transitions between the steps of forward and reverse splicing of group IIC introns

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FIGURE 3.
FIGURE 3.

Ta.it.I1 in vitro splicing activity. (A) Ta.it.I1 splicing assay under buffer conditions of 0.5 M NH4Cl and [MgCl2] ranging from 5–100 mM, with and without addition of IEP at 55°C. (B) Comparison of Ta.it.I1 splicing in 100–500 mM NH4Cl and KCl buffers. (C) Ta.it.I1 intron splicing assay under 0.5 M NH4Cl and 10 mM MgCl2 buffer at incubation temperatures from 45°C–75°C. (D) Ta.it.I1 intron splicing assay with WT or YAAA mutant IEP.

This Article

  1. Published in Advance March 3, 2020, doi: 10.1261/rna.075044.120 RNA 26: 664-673

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