Transitions between the steps of forward and reverse splicing of group IIC introns

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FIGURE 2.
FIGURE 2.

Reconstitution of Ta.it.I1 IEP and precatalytic intron RNA. (A) Schematic representation of the Ta.it.I1 IEP purification construct and SDS-PAGE of purified IEP. Mutations of the IEP used in this study are shown. (B) Fluorescent reverse transcription assay of WT and YAAA mutant IEP. (C) 4% PAGE analysis of Ta.it.I1 intron in vitro transcription products under various [MgCl2].

This Article

  1. Published in Advance March 3, 2020, doi: 10.1261/rna.075044.120 RNA 26: 664-673

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