MBNL2 controls the hypoxia-dependent alternative splicing of CE. (A) Venn diagram showing the overlap of AS changes after knockdown of MBNL2 and in response to hypoxia in MCF-7. Not significant (Fisher's exact test, one-tailed). The number in brackets refers to AS events regulated in the opposite direction. (B) Scatter plot comparing the fold changes of shared AS events after knockdown of MBNL2 and in response to hypoxia in MCF-7. Pearson correlation coefficient and associated P-value are given. (C) RT-PCR of PIGN exon 12 in normoxic and hypoxic MCF-7 cells and after knockdown of MBNL2. The short isoform (100 bp) corresponds to exon 2 exclusion, the long isoform (160 bp) to exon 12 inclusion. n = 2. (M) Size marker.
