MBNL2 is specifically induced by chronic hypoxia and contributes to the adaptation of cancer cells. (A) RT-qPCR quantification of the mRNA levels of MBNL1 and MBNL2 in normoxic and hypoxic A549 and MCF-7 cells. Values are normalized to the housekeeping gene RPLP0. n = 4. (B) Western blot of MBNL1 and MBNL2 in normoxic and hypoxic A549 and MCF-7 cells. Anti-MBNL1 and anti-MBNL2 antibodies were used to visualize the respective protein levels. Total lane protein is shown as loading control. n = 2. (N) Normoxia, (H) Hypoxia. (C) Cell viability of hypoxic A549 and MCF-7 cells after knockdown of MBNL2. Cells were additionally treated with the indicated concentrations of cisplatin for 24 h. Absorption was normalized to normoxic control cells with the respective cisplatin concentration. n = 3. (D) Migration assay of hypoxic A549 cells after knockdown of MBNL2. As positive control, cells were stimulated with 100 ng/µL hEGF. n = 3. (E) Representative pictures used to quantify the numbers of migrated A549 cells in D. (**) P < 0.01, (*) P < 0.05.
