Quantitating the flexibility of HOTAIR. (A) A range of HOTAIR morphologies, segment names are abbreviated. Left panel shows HOTAIR interacting with HBD1 through the U-module (left panel bar = 50 nm; other panels bar = 20 nm). (B) Fast AFM scan of HOTAIR, tracking the movement of segments HS (blue arrowheads) and LS (white arrowheads; bar = 20 nm). (C) Fast AFM scan of HOTAIR focusing on movements of the U-module, exhibiting pincer-like opening/closure and a joint in segment UL (bar = 25 nm). (D) Quantitative analysis of the displacement (nm) of HL/HS movements. Numbers were corrected to account for center-mass drift. (E) Quantitative analysis of U-module behavior (0 nm represents closed state; other numbers represent varying levels of opening).
