Summary of the results of translation assays with chimeric RNAs. (A) Poly(A)-dependent stimulation. Stimulation factors were calculated from pairwise comparisons to the bare nLuc RNA as indicated in Figure 3B,C (P = 0.04 for forward-long-SRE− RNA and P ≤ 1.6 × 10−4 for all others). SRE+ RNAs were not considered, as explained in the text. (B) SRE-dependent repression. Numbers were calculated from pairwise comparisons of SRE+ and SRE− variants as shown in Figure 3C (P ≤ 1.3 × 10−4 for all RNAs). All data in A and B are presented both corrected for translation efficiency in RRL (red bars) and without correction (blue bars). Correction factors in individual experiments were at most 2 and typically lower. Error bars indicate the standard deviation based on the numbers of independent experiments reported in Figure 3A. The statistical significance of the pairwise comparisons indicated by brackets is indicated by (***) (P < 0.001) and ns (nonsignificant).
