EVE-derived piRNAs from the flamenco-like locus associate with mosquito PIWI proteins. (A) Size profile of small RNA derived from miRNAs or the flamenco-like locus in control or oxidation-treated libraries. The nt at the 5′ end is indicated by color. (B) Association of piRNAs derived from the top five uni- and dual-strand clusters (highlighted in Supplemental Table S4) compared to microRNAs with different PIWI proteins based on immunoprecipitations from mosquito Aag2 cells. The flamenco-like locus belongs to the group of uni-strand piRNA clusters and is highlighted in red for comparison. Statistics were performed using Wilcoxon rank-sum test. Significant P-values (P < 0.01) are indicated. Small RNA libraries from mosquito Aag2 cells were used in these analyses.
