Secondary structures of the HTLV-1 pro-pol frameshift site variants. The data-driven secondary structures of the HTLV-1 pro-pol (A) ΔPK1, (B) PKM1, (C) PKM2, and (D) PKM3 frameshift site variants are shown. On the left side of the panel, the normalized and averaged NMIA nucleotide (NT) reactivities are superimposed on the structures. Percentages shown represent helix-wide confidence values determined with bootstrapping methods. On the right side of the panel, normalized and averaged A and C NT reactivities (derived from experiments with DMS) are overlaid onto each structure. All sequence changes from WT are shown in red font. Note that 2 bp predicted in the loops of ΔPK1 and PKM1 between nucleotides A2268–U2276 and C2269–G2275 were not included in the figures. (Figure continues on next page.)
