Ppp1r1b-lncRNA inhibits PRC2 at myogenic regulatory genes to promote cardiac and skeletal muscle development in mouse and human

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 6.
FIGURE 6.

Ppp1r1b-lncRNA binds EZH2. (A) RNA pull-down assays were carried out by incubating cell lysate from neonatal mouse heart tissue with biotinylated in vitro transcribed Ppp1r1b-lncRNA. Subsequent western blotting analysis revealed EZH2 in the isolated RNA-protein complex. (B) RNA immunoprecipitation (RIP) demonstrated that Ppp1r1b-lncRNA was immunoprecipitated together with EZH2 in neonatal mouse heart tissue. LncRNA was detected using real-time PCR. (*) P < 0.05.

This Article

  1. Published in Advance January 17, 2020, doi: 10.1261/rna.073692.119 RNA 26: 481-491

Current Issue

  1. July 2026, 32 (7)
  1. Current Issue
  1. Alert me to new issues of RNA