Ppp1r1b-lncRNA inhibits Histone 3 methylation at the promoter regions of myogenic regulatory factors. C2C12 myoblasts were cultured in growth medium (GM) or differentiation medium (DM) and treated by scramble GapmeR or Ppp1r1b-lncRNA GapmeR. After treatment, cells were subjected to ChIP analysis using anti H3K27me3 antibodies. The qPCR data are presented as fold enrichment to the background, indicating the abundance of histone modifications at the promoter regions of the myogenic genes. (A) H3K27me3 level on MyoD1 promoter. (B) H3K27me3 level on Myogenin promoter. (C) Western blot analysis of myogenic regulatory factors and sarcomeric structural proteins from C2C12. Gapdh was used as a loading control. (GM) Growth medium, (DM) differentiation medium. (*) P < 0.05; (**) P < 0.01.
