Ppp1r1b-lncRNA inhibits PRC2 at myogenic regulatory genes to promote cardiac and skeletal muscle development in mouse and human

Human PPP1R1B-lncRNA promotes differentiation of human iPSC-derived cardiomyocyte (hiPSC-CM). (A) Microscope images depict the differentiation efficiency of human hiPSC-CM (Myosin positive cells) after PPP1R1B-lncRNA siRNA, or Scramble, treatment. (WGA) Wheat germ agglutinin. Scale bar, 100 µm. (B) Quantitative real-time PCR analysis of PPP1R1B-lncRNA expression after siRNA treatment. (C) Quantitative analysis depicts differentiation efficiency of hiPSC-CMs after siRNA treatment. (EB) Embryonic bodies. N = 4 biological replicates per condition; error bars, standard error of the mean; (*) P < 0.05; (**) P < 0.01.