Ppp1r1b-lncRNA inhibits PRC2 at myogenic regulatory genes to promote cardiac and skeletal muscle development in mouse and human

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FIGURE 2.
FIGURE 2.

Human PP1R1B-lncRNA promotes differentiation of human skeletal myoblast. (A) Fluorescent microscope images depict inhibition of myogenesis of human skeletal myoblast (myosin positive cells) after PPP1R1B-lncRNA siRNA treatment. Scale bar, 100 µm. (B) Higher magnification images of cells presented in A. (C) Real-time PCR analysis and fusion index analysis of human myoblast before and after differentiation (625 nuclei counted for growth medium; 571 nuclei counted for differentiation medium). (D) RNA fractionation analysis of PPP1R1B-lncRNA down-regulated by siRNA. (E) Real-time PCR analysis and fusion index analysis of human myoblast after PPP1R1B-lncRNA siRNA treatment in differentiation medium (737 nuclei counted for Scramble; 760 nuclei counted for siRNA). N = 4 biological replicates per condition; error bars, standard error of the mean; (*) P < 0.05; (**) P < 0.01.

This Article

  1. Published in Advance January 17, 2020, doi: 10.1261/rna.073692.119 RNA 26: 481-491

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