Ppp1r1b-lncRNA inhibits PRC2 at myogenic regulatory genes to promote cardiac and skeletal muscle development in mouse and human

Ppp1r1b-lncRNA is induced during myogenesis and regulates C2C12 myoblast differentiation. (A,C) Quantitative real-time PCR analysis of Ppp1r1b-lncRNA expression after 2 d of differentiation (A) and GapmeR treatment (C). (B) Subcellular localization of Ppp1r1b-lncRNA in myoblasts. U6 RNA was used as a nuclear location control. (D) Light microscope images depict the morphology of C2C12 cells after 2 d of differentiation and Ppp1r1b-GapmeR, or Scramble, treatment. Scale bar, 200 µm. N = 4 biological replicates per condition; error bars, standard error of the mean; (**) P < 0.01.